millicell-cm slice culture inserts (Millipore)

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Millipore millicell-cm slice culture inserts
Millicell Cm Slice Culture Inserts, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/millicell-cm+slice+culture+inserts/millicell+inserts/pm40659844-372-4-8
Average 90 stars, based on 1 article reviews

millicell-cm slice culture inserts - by Bioz Stars, 2026-09

90/100 stars

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Article Title: Extensive migration of young neurons into the infant human frontal lobe
Article Snippet: The sections were then transferred to Millicell-CM slice culture inserts (Millipore) that were immersed in cortical slice culture medium [66% BME, 25% Hanks, 5% FBS, 1% N-2, 1% penicillin, streptomycin, and glutamine (all Invitrogen) and 0.66% D-(+)-glucose (Sigma-Aldrich)].

Microscopy:

Article Title: Blood vessels guide Schwann cell migration in the adult demyelinated CNS through Eph/ephrin signaling
Article Snippet: .. Spinal cord segments including lesion and graft sites were laid onto Millicell-CM slice culture inserts (Millipore) over culture medium (50% DMEM + Glutamax, 25% HBSS, 25% heat-inactivated horse serum, 5 mg/mL D-(+)-glucose, 20 mM Hepes, penicillin (100 mg/ml), streptomycin (100 U/ml), hrNDFß (125 ng/ml), and forskolin (2 μg/ml) in glass bottom plates, and then placed in an inverted Leica SP8X confocal microscope with an on-stage incubator, while streaming 95% O 2 and 5% CO 2 into the chamber. ..

Article Title: Blood vessels guide Schwann cell migration in the adult demyelinated CNS through Eph/ephrin signaling.
Article Snippet: .. Spinal cord segments including lesion and graft sites were laid onto Millicell-CM slice culture inserts (Millipore) over culture medium (50% DMEM + Glutamax, 25% HBSS, 25% heat-inactivated horse serum, 5 mg/mL D-(+)-glucose, 20 mM Hepes, penicillin (100 mg/ml), streptomycin (100 U/ ml), hrNDFß (125 ng/ml), and forskolin (2 μg/ml) in glass bottom plates, and then placed in an inverted Leica SP8X confocal microscope with an on-stage incubator, while streaming 95% O2 and 5% CO2 into the chamber. ..

Modification:

Article Title: An expanded subventricular zone supports postnatal cortical interneuron migration in gyrencephalic brains.
Article Snippet: Brains were embedded in 3.5% low-melting-point agarose (Thermo Fisher Scientific, BP165-25) and sectioned using a Leica VT1200S vibrating blade microtome as 300 μm slices (speed, 2 mm s−1; amplification 0.8 mm). .. Slices were transferred into Millicell-CM slice culture inserts (Millipore, PICM03050) that were immersed in the modified DMEM/F12 + GlutaMAX-I culture medium (Gibco, 0565018) with 1× N2 supplement (Gibco, 17502048), 0.05× B27 supplement (Thermo Fisher Scientific, 17504044), 20 ng ml−1 hFGF-2 (Gibco, 13256029), 20 ng ml−1 hEGF (Gibco, PHG0311), 20 μg ml−1 human insulin (Sigma-Aldrich, I9278), 5 ng ml−1 BDNF (Sigma-Aldrich, SRP3014), 10 μM Rock inhibitor Y-27632 (Stemgent, 04-0012) and 100 U ml−1 penicillin–streptomycin (Gibco, 15140-122). .. Tissue slices were microinjected with the virus (Ad-CMV-GFP, 1 × 1010 PFU ml−1, 1 μl; AAV2-CAG-GFP, 1 × 1013 GC ml−1, 1 μl; Vector Biolabs) into Arc and were kept at 37 °C with 5 % CO2 and 8% O2.

Cell Culture:

Article Title: Outer Radial Glia-like Cancer Stem Cells Contribute to Heterogeneity of Glioblastoma.
Article Snippet: Brain tissue was embedded in 3% lowmelting point agarose in ACSF and 250–300 mm coronal vibratome sections were generated. .. Sections were transferred on Millicell-CM slice culture inserts (Millipore) and cultured in cortical slice culture medium (66% BME, 25% Hanks, 5% FBS, 1% N-2, 1% penicillin, streptomycin and glutamine, all Invitrogen, and 0.66% d-(+)-glucose, Sigma-Aldrich). .. High titer gfp labeled lentivirus was applied directly to slices that were then cultured at 37 C, 5%CO2, 8% O2 for 96 h. For time-lapse imaging, cultures were then transferred to an inverted Leica TCS SP5with an on-stage incubator streaming 5%CO2, 5%O2, balance N2 into the chamber.

Incubation:

Article Title: Aberrant oligodendroglial-vascular interactions disrupt the Blood Brain Barrier triggering CNS inflammation
Article Snippet: .. Slices were transferred onto Millicell-CM slice culture inserts (Millipore) over culture medium (FluoroBrite DMEM with 25% horse serum, 25% HBSS, 1% N2 supplement, 1% penicillin and streptomycin) in glass bottom 6-well plates and incubated at 37 °C, 5% CO2 for at least 1 hour. .. The plate was then transferred to an inverted Leica TCS SP5X confocal with an on-stage incubator (while streaming 5% CO2, 5% O2), and imaged using a 10x immersion objective (NA=0.3) at specified interval and total imaging time in texts with intermittent repositioning of the focal planes.

Article Title: Multimodal Single-Cell Analysis Reveals Physiological Maturation in the Developing Human Neocortex
Article Snippet: .. Slices were transferred to and suspended on Millicell-CM slice culture inserts (Millipore) over culture medium (66% BME, 25% Hanks, 5% FBS, 1% N-2, 1% penicillin, streptomycin, and glutamine (all Invitrogen) and 0.66% d-(+)-glucose (Sigma)) in 6-well plates and incubated at 37°C, 8% O2, 5% CO2 for 2 days. .. For morphology assays, slices were incubated with Ad-CMV-GFP virus (1:2000, Vector Biolabs, 1060) for 30 min prior to positioning on slice culture inserts.

Imaging:

Article Title: Dynamic behaviour of human neuroepithelial cells in the developing forebrain.
Article Snippet: .. For live imaging studies, 250mm coronal vibratome sections were transferred to Millicell-CM slice culture inserts (Millipore) immersed in cortical slice culture medium (60% BME, 25% Hanks, 10% FBS, 1% N-2, 1% B-27, 1% penicillin, streptomycin and glutamine, all Invitrogen, and 0.66% d-(þ )-glucose, Sigma-Aldrich). .. CMV-GFP adenovirus at a dilution of 1:50–1:500 (Vector Biolabs, 1 106 colony forming units) was applied to slices that were then cultured at 37 C, 5% CO2, 8% O2 for 12-30 h. For timelapse imaging, cultures were then transferred to an inverted Leica TCS SP5 with an on-stage incubator streaming 5% CO2, 8% O2, balance N2 into the chamber.

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